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Naga figures, which include Vasuki and Takshaka, also play pivotal roles in different legends, including the churning in the ocean of milk as well as the epic battles while in the Mahabharata. Symbolism and Worship

Weaving can be an intricate and time consuming work and each shawl can take at the very least a few days to accomplish. Patterns for shawls and wraparound garments (typically called mekhala) are various for men and girls.

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The loss of exercise is just not due to a diminished capacity to bind divalent cations since the metallic articles with the purified mutants was identical to the wild sort enzyme. The catalytic Homes of those mutants are thoroughly according to the proposed purpose of the residue while in the chemical mechanism as the primary general foundation for activation with the hydrolytic water molecule and the next protonation of your leaving team amine.

From three g of cell paste about a hundred mg of homogeneous protein ended up isolated. Enzymatic exercise was dependent on the existence of disulfide reducing reagents which include dithiothreitol or β-mercaptoethanol throughout storage and purification. The native molecular body weight in the purified enzyme was believed to become one hundred eighty kDa based mostly slot naga26 upon the elution volume via a calibrated gel filtration column (knowledge not demonstrated).

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An historic tomb preserved among the Bicolanos, learned and examined by anthropologists in the course of the 1920s, refers to some of the exact same deities and personages talked about within the Maragtas.[21]

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Incubation from the apo-enzyme with one equal of Zn2+ for half-hour absolutely restored catalytic activity. The titration of apo-enzyme with different amounts of ZnCl2 is shown in Figure 1. These results display that NagA from E. coli